Summary of IPLab Features
| Multi-Fluorescence: | Capture images from multiple wavelengths, overlay colors, and quantify intensities. |
| Time Lapse: | Capture images at specified time intervals. Measure and plot intensities over time. |
| Normalization: | Enhance brightness and contrast, and apply a gamma curve to the displayed image data. |
| Batch File Processing: | Create a list of files to process all at once. Indexed files provide automatic file naming and numbering. |
| Animation: | Create a movie loop from your time lapse and 3D images. Play it in IPLab or export it as movie files: QuickTime on the Mac and AVI on Windows. |
| Merge Color Channels: | Build a 24- or 48-bit color image out of three separate image files or sequences. Blend a fluorescent image or sequence with a DIC or other grayscale image. |
| Split Color Channels: | Split any 24- or 48-bit color image into the separate components. View the separated images in their corresponding colors. Operate on the color component images as you would any grayscale image. |
| Shading Correction: | Easily perform flat-fielding to correct for lighting and sensor non-uniformities. |
| Segment Morphology: | Erosion, dilation, opening, and closing filter operations, with user-definable kernels. |
| Enhance: | Choose from built-in sharpening, smoothing, and median filters; pseudocolor tables, contrast enhancements, and tools for geometric re-sizing. |
| Custom Filters: | Define your own linear filter kernels. |
| Cut, Copy, Paste: | Exchange image data with other programs through cut-and-paste. |
| Image Registration: | Add registration marks to several images and let IPLab automatically rotate, scale, and shift the images to bring them into alignment. |
| Image Arithmetic: | Combine and compare two images on a pixel-by-pixel basis. Perform background subtraction and image averaging to reduce noise. Mask certain frequencies in the Fourier domain. |
| Edit Color Table: | Complete control over the color lookup table to pseudo-color images, equalize histograms, and stretch the image contrast. |
| Geometric Transforms: | Arbitrary rotation, scale and translation with sub-pixel accuracy. Choose nearest neighbor or bilinear interpolation methods. Also transpose and flip images to view them differently. |
| Complex Arithmetic: | Complex multiplication of Fourier spectrum real and imaginary components. Convert between real-imaginary and magnitude-phase. |
| FFT and Cosine Transformations: | Forward and inverse 1-D and 2-D Fast Fourier transform with results in either real-imaginary or magnitude-phase. Forward and inverse cosine transform. All transformations are done with floating-point values for best accuracy. |
| Point Functions: | Choose from an extensive list of built-in mathematical operations to apply to each pixel for analysis and enhancement. |
| Quantify: | Count objects and measure density, shape, position, and intensity moments for individual objects and for groups. Limit measurements to specific regions and/or value ranges. Automatically label measured items. Export results to spreadsheets. |
| Image Ratios: | Compute optical density, perform accurate ratios of images taken at different wavelengths. |
| Row/Column Averages: | Plot the average value or the sum of all the pixels along a row or column of a rectangular or polygonal region. |
| Calibrate Units: | Calibrate measurements in units of your choice-- microns, mm, cm, inches, etc. |
| Segmentation / Thresholding: | Interactively select thresholds to separate objects from their background. |
| Densitometry: | Integrated and mean densities, RMS and standard deviation, max and min values. |
| Histogram: | Plot image data using color histograms. Customizable or automatic binning. |
| Slices and Object Boundaries: | Plot data values along a slice through the image, or list the (x,y) coordinates and data values at each point along the boundary of an object. |
| Graphs and Plots: | Our integrated graphing functions produce publication quality graphs of your data and analysis results. |
| Measurements: | IPLab has a long list of measurements you can perform, for measuring density, shape, position, and intensity moments. Limit measurements to specific regions and/or value ranges. |
| Interactive Measurement: | Click the mouse to quickly measure lengths and angles. Automatically creates table of measurements and marks the measured lines and angles on the image within a nondestructive overlay. |
| Scripts and Macros: | Record your own macros to automate your experiments. Run reproducible protocols as if they were a single command. Assign your most common scripts to keyboard function keys. Turn complex procedures into a sequence of labeled key-presses. There is no scripting language to learn; just point and click. |
| Set Pattern: | Add or replace values to the region of interest: horizontal and vertical ramps, Gaussian and uniform random noise. |
| Custom Extensions: | Customize IPLab further by programming your own algorithms. The manual gives complete instructions and examples for including your own code and dialogs written using CodeWarrior for Macintosh and Microsoft Visual C++TM. |
| Mosaic: | Capture multiple images and place them together in one large montage. |
| Image Annotation: | Non-destructive overlays let you draw text and graphics on top of your images without altering the underlying data values. IPLab saves both the overlay and image data. |
| Transfer Attributes: | Easily transfer information from one image to another, including the ROI and object definitions, color table, overlays, and units. |
| Opening and Saving Images | Read and write files in a number of formats: TIFF, Text, and our own IPLab formats from both Mac and Windows. IPLab/Mac also handles PICTs, FITS, and EPR (*.raw and *.psf) formats. IPLab's powerful Open As command imports foreign files in almost any file format. |
| Export to Excel®: | Easily export results to Microsoft Excel®. In IPLab for Windows, use our Export to Excel command to open your data in Microsoft Excel 2002 or later. In any version of IPLab, you can save your data as text and easily open it in Excel or any other spreadsheet program. |
| Print: | Print images as halftones to any PostScript printer or QuickDraw compatible printer. Make a PostScript file of your image data. |
| View As Text: | Switch easily between a text view and a standard image view of your data. |
Continue Reading
Please continue reading about IPLab:
- IPLab : The main page of information about the IPLab program
- Extensions : Describes software that adds abilities to IPLab
- System Requirements : The minimum computer systems needed to run IPLab